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TIM3 Polyklonaler Antikörper

TIM3 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11872-1-PBS

Synonyme

CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3

Filter:
  • Western Blot (WB) analysis of various lysates using TIM3 Polyclonal antibody (11872-1-AP)

    WB analysis using 11872-1-AP (same clone as 11872-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11872-1-AP (TIM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TIM3 Polyclonal antibody (11872-1-AP)

    IHC staining of human tonsillitis using 11872-1-AP (same clone as 11872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11872-1-AP (TIM3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11872-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TIM3 Polyclonal antibody (11872-1-AP)

    IF Staining of human tonsillitis using 11872-1-AP (same clone as 11872-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TIM3 antibody (11872-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11872-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11872-1-PBS bindet in WB, IHC, IF-P, ELISA TIM3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TIM3 fusion protein Ag2459
Vollständiger Name hepatitis A virus cellular receptor 2
Berechnetes Molekulargewicht 301 aa, 33 kDa
Beobachtetes Molekulargewicht 50-70 kDa, 33 kDa
GenBank-ZugangsnummerBC020843
Gene symbol TIM3
Gene ID (NCBI) 84868
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301)