TIM3 Rekombinanter Antikörper

TIM3 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLux Violet 510 Fluorescent Dye

CloneNo.

241422H1

Kat-Nr. : CLV510-98115

Synonyme

241422H1, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3

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  FC experiment of human PBMCs using CLV510-98115

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with 5 ul CoraLux Violet 510 Anti-Human TIM3 Rabbit RecAb (CLV510-98115, Clone: 241422H1) and 5 ul CoraLite® Plus 488 Anti-Human CD4 Rabbit Recombinant Antibody (CL488-98042, Clone: 240427E12). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CLV510-98115 bindet in FC TIM3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: hepatitis A virus cellular receptor 2
• Berechnetes Molekulargewicht: 301 aa, 33 kDa
• GenBank-Zugangsnummer: BC020843
• Gene symbol: TIM3
• Gene ID (NCBI): 84868
• Konjugation: CoraLux Violet 510 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 410 nm / 501 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920).