TIMELESS Monoklonaler Antikörper

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• KD/KO Validated

TIMELESS Monoklonal Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B6G3

Kat-Nr. : 67022-1-PBS

Synonyme

hTIM, Protein timeless homolog, TIM, TIM1, timeless homolog (Drosophila)

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  WB analysis using 67022-1-Ig (same clone as 67022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67022-1-Ig (same clone as 67022-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of JAR using 67022-1-Ig (same clone as 67022-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67022-1-Ig (same clone as 67022-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67022-1-Ig (same clone as 67022-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67022-1-Ig (same clone as 67022-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67022-1-Ig (same clone as 67022-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 67022-1-Ig (same clone as 67022-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human TIMELESS (67022-1-Ig, Clone:1B6G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67022-1-PBS bindet in WB, FC (Intra), Indirect ELISA TIMELESS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TIMELESS fusion protein Ag6283
• Vollständiger Name: timeless homolog (Drosophila)
• Berechnetes Molekulargewicht: 139 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC050557
• Gene symbol: TIMELESS
• Gene ID (NCBI): 8914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.