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TIM10 Polyklonaler Antikörper

TIM10 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 11124-2-AP

Synonyme

TIMM10, TIM10A, TIMM10A

Filter:
  • Western Blot (WB) analysis of various lysates using TIM10 Polyclonal antibody (11124-2-AP)

    WB analysis using 11124-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11124-2-AP (TIM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TIM10 Polyclonal antibody (11124-2-AP)

    IF Staining of HepG2 using 11124-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIM10 antibody (11124-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen, MDA-MB-231-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 3 publications below

Produktinformation

11124-2-AP bindet in WB, ELISA TIM10 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TIM10 fusion protein Ag1615
Vollständiger Name translocase of inner mitochondrial membrane 10 homolog (yeast)
Berechnetes Molekulargewicht 10 kDa
Beobachtetes Molekulargewicht 10 kDa
GenBank-ZugangsnummerBC032133
Gene symbol TIMM10
Gene ID (NCBI) 26519
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TIM10 antibody 11124-2-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh
View Article
ratWB

Redox Biol

Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.

Authors - Wei Ou
View Article
humanWB

PLoS One

Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV.

Authors - Rui Huang
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Wenhui (Verified Customer) (01-02-2025)

This antibody has been working great with WB and gives well define band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiac tissue and cardiomyocytes