TIM23 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TIM23 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(156)

Rezensionen (2)

Kat-Nr. : 11123-1-AP

Synonyme

TIMM23, Mitochondrial import inner membrane translocase subunit Tim23, TIMM 23

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  WB analysis using 11123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11123-1-AP (Tim23 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 11123-1-AP

  IP result of anti-Tim23 (IP:11123-1-AP, 4ug; Detection:11123-1-AP 1:1000) with mouse heart tissue lysate 1200 ug.

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  IHC staining of mouse testis using 11123-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11123-1-AP (TIM23 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11123-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11123-1-AP (TIM23 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11123-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIM23 antibody (11123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 143 publications below
• IHC: See 3 publications below
• IF: See 16 publications below
• IP: See 1 publications below

Produktinformation

11123-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TIM23 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, hamster, Hausschwein, Hund, Kaninchen, Maus, Ratte, Rind, Riesenscheibenbrasse (Megalobrama amblycephala)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIM23 fusion protein Ag1620
• Vollständiger Name: translocase of inner mitochondrial membrane 23
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC062707
• Gene symbol: TIMM23
• Gene ID (NCBI): 100287932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIM23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

Publikationen

Species	Application	Title
mouse	IF	Nature; Cellular ATP demand creates metabolically distinct subpopulations of mitochondria; Authors - Keun Woo Ryu
mouse	WB	Nature; CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization; Authors - Kevin Qian
mouse	WB	Nature; The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.; Authors - Atsushi Hoshino
human	WB	Nature; In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.; Authors - Milica Momcilovic
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wenhui (Verified Customer) (01-02-2025)

This antibody is good for detecting correct and clear band for WB with cardiac tissues and cardiomyocytes. I would have recommended it.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiac tissues and cardiomyocytes

FH

Angela (Verified Customer) (12-27-2024)

very good antibody , purchase many times!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart tissue

TIM23 Antibody Western Blot validation (1:1000 dilution) in heart tissue (Cat no:11123-1-AP)