TIMM44 Monoklonaler Antikörper

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• KD/KO Validated

TIMM44 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2c

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4G7D4

Rezensionen (1)

Kat-Nr. : 66149-1-Ig

Synonyme

4G7D4, MIMT44, TIM44, TIMM 44

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  WB analysis using 66149-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66149-1-Ig (TIMM44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HeLa using 66149-1-Ig

  WB result of TIMM44 antibody (66149-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIMM44 transfected HeLa cells.

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  IHC staining of human liver using 66149-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66149-1-Ig (TIMM44 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66149-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66149-1-Ig (TIMM44 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66149-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIMM44 antibody (66149-1-Ig, Clone: 4G7D4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, Hausschwein-Herzgewebe, Kaninchenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66149-1-Ig bindet in WB, IHC, IF/ICC, ELISA TIMM44 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2c
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TIMM44 fusion protein Ag21389
• Vollständiger Name: translocase of inner mitochondrial membrane 44 homolog (yeast)
• Berechnetes Molekulargewicht: 452 aa, 51 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC033628
• Gene symbol: TIMM44
• Gene ID (NCBI): 10469
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Translocase of inner mitochondrial membrane 44 (TIMM44), also known as MIMT44 and TIM44, belongs to the Tim44 family. TIMM44 is a mitochondrial protein located at the inner mitochondrial membrane, which is crucial for the integrity and function of mitochondria. In an ATP-dependent manner, TIMM44 anchors mitochondrial heat shock protein 70 to the translocase of the mitochondrial inner membrane 23 (TIMM23) complex. TIMM44 is essential for mitochondrial pre-protein import into the mitochondrial matrix. TIMM44 is vital for the integrity and function of mitochondria. TIMM44 silencing disrupted mitochondrial functions in endothelial cells, causing mitochondrial protein input arrest, ATP reduction, ROS production, and mitochondrial depolarization, and leading to apoptosis activation (PMID: 36438483, PMID: 37147302, PMID: 38467612). The observed molecular weight of TIMM44 is 45 kDa, indicating a mature form of TIMM44 (PMID: 33891006).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lana (Verified Customer) (04-20-2019)

SDS-PAGE:15 ug/ul RIPA lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 800CW Goat anti-Mouse1 h incubation at room temperatureLines on WB:1. BioRad Precision Plus Protein standards2. Lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ST-Hdh mouse striatal cell line

TIMM44 Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:66149-1-Ig)