TIMM44 Monoklonaler Antikörper

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TIMM44 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2c

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4G7D4

Kat-Nr. : 66149-1-PBS

Synonyme

4G7D4, MIMT44, TIM44, TIMM 44

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  WB analysis using 66149-1-Ig (same clone as 66149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66149-1-Ig (TIMM44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66149-1-Ig (same clone as 66149-1-PBS)

  WB result of TIMM44 antibody (66149-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIMM44 transfected HeLa cells. This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66149-1-Ig (same clone as 66149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66149-1-Ig (TIMM44 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66149-1-Ig (same clone as 66149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66149-1-Ig (TIMM44 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66149-1-Ig (same clone as 66149-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIMM44 antibody (66149-1-Ig, Clone: 4G7D4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66149-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66149-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TIMM44 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2c
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TIMM44 fusion protein Ag21389
• Vollständiger Name: translocase of inner mitochondrial membrane 44 homolog (yeast)
• Berechnetes Molekulargewicht: 452 aa, 51 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC033628
• Gene symbol: TIMM44
• Gene ID (NCBI): 10469
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.