TIMP1 Rekombinanter Antikörper

TIMP1 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230329E7

Kat-Nr. : 83151-5-PBS

Synonyme

TIMP-1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, Collagenase inhibitor

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  IF Staining of HeLa using 83151-5-RR (same clone as 83151-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83151-5-RR (same clone as 83151-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HT-29 using 83151-5-RR (same clone as 83151-5-PBS)

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

• 

  FC experiment of MDA-MB-231 using 83151-5-RR (same clone as 83151-5-PBS)

  1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83151-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83151-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83151-5-RR against Human TIMP1 were performed. The affinity constant is 1.33 nM.

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Geprüfte Anwendungen

Produktinformation

83151-5-PBS bindet in IF/ICC, FC (Intra), ELISA TIMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: TIMP metallopeptidase inhibitor 1
• Berechnetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC000866
• Gene symbol: TIMP1
• Gene ID (NCBI): 7076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.