TINAGL1 Polyklonaler Antikörper

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• KD/KO Validated

TINAGL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12077-1-PBS

Synonyme

PP6614, Oxidized LDL-responsive gene 2 protein, OLRG-2, LCN7, Glucocorticoid-inducible protein 5

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  WB analysis using 12077-1-AP (same clone as 12077-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

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  WB analysis of human adrenal gland using 12077-1-AP (same clone as 12077-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 12077-1-AP (same clone as 12077-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 12077-1-AP (same clone as 12077-1-PBS)

  IP result of anti-TINAGL1 (IP:12077-1-AP, 4ug; Detection:12077-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12077-1-AP (same clone as 12077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12077-1-AP (same clone as 12077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 12077-1-AP (same clone as 12077-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TINAGL1 antibody (12077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12077-1-AP (same clone as 12077-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TINAGL1 antibody (12077-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12077-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA TINAGL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TINAGL1 fusion protein Ag2721
• Vollständiger Name: tubulointerstitial nephritis antigen-like 1
• Berechnetes Molekulargewicht: 467 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC009048
• Gene symbol: TINAGL1
• Gene ID (NCBI): 64129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tubulointerstitial nephritis antigen-like 1 (Tinagl1), a secreted extracellular protein, was initially identified as a putative component of the ECM. Tinagl1 is most commonly associated with embryonic development, including the heart and adrenal glands. Low levels of Tinagl1 expression can be dire for craniofacial development and for female fertility.