TINP1 Monoklonaler Antikörper

TINP1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E3B11

Kat-Nr. : 66374-1-PBS

Synonyme

HUSSY-29, HUSSY29, HCL G1, Hairy cell leukemia protein 1, Hairy cell leukemia

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  WB analysis of L02 using 66374-1-Ig (same clone as 66374-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66374-1-Ig (same clone as 66374-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66374-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TINP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TINP1 fusion protein Ag8822
• Vollständiger Name: TGF beta-inducible nuclear protein 1
• Berechnetes Molekulargewicht: 260 aa, 30 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC005288
• Gene symbol: TINP1
• Gene ID (NCBI): 10412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.