Perilipin 3/TIP47 Monoklonaler Antikörper

Perilipin 3/TIP47 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4C11B1

Kat-Nr. : 66523-1-PBS

Synonyme

M6PRBP1, Perilipin 3, TIP47, Mannose-6-phosphate receptor-binding protein 1, 4C11B1

---

• 

  WB analysis using 66523-1-Ig (same clone as 66523-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 66523-1-Ig (same clone as 66523-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66523-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Perilipin 3/TIP47 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Perilipin 3/TIP47 fusion protein Ag1028
• Vollständiger Name: mannose-6-phosphate receptor binding protein 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC007566
• Gene symbol: TIP47/PLIN3
• Gene ID (NCBI): 10226
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.