TIPIN Polyklonaler Antikörper

TIPIN Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11972-2-AP

Synonyme

TIMELESS interacting protein, TIPIN

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  WB analysis using 11972-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11972-2-AP (TIPIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11972-2-AP bindet in WB, IHC, ELISA TIPIN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIPIN fusion protein Ag2595
• Vollständiger Name: TIMELESS interacting protein
• Berechnetes Molekulargewicht: 301 aa, 34 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000870
• Gene symbol: TIPIN
• Gene ID (NCBI): 54962
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIPIN, also named as TIMELESS-interacting protein, is a 301 amino acid protein, which belongs to the CSM3 family. TIPIN Plays an important role in the control of DNA replication and the maintenance of replication fork stability. TIPIN forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. The calculated molecular weight of TIPIN is 34 kDa, but the result of WB is 55-60 kDa, which is similar to the result of publication.(PMID: 24830473)

Publikationen

Species	Application	Title
human	IHC	Dis Markers; Integrated Bioinformatic Analysis Identifies TIPIN as a Prognostic Biomarker in Hepatocellular Carcinoma.; Authors - Hui Chen
human	WB	Nature; Mechanism for local attenuation of DNA replication at double-strand breaks; Authors - Robin Sebastian ; KD Validated