Transketolase Monoklonaler Antikörper

Transketolase Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

3E2F3

Kat-Nr. : 66016-1-Ig

Synonyme

TK, TKT, TKT1, Transketolase

---

Galerie der Validierungsdaten

• 

  WB analysis using 66016-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66016-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66016-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 66016-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66016-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66016-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66016-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66016-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 66016-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 66016-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 66016-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66016-1-Ig(Transketolase antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66016-1-Ig

  1X10^6 HepG2 cells were stained with 0.2ug Transketolase antibody (66016-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Lebergewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66016-1-Ig bindet in WB, IF, FC, ELISA Transketolase und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Transketolase fusion protein Ag16779
• Vollständiger Name: transketolase
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC009970
• Gene symbol: TKT
• Gene ID (NCBI): 7086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

Publikationen

Species	Application	Title
human	WB	Cancer Med; Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma; Authors - Yimin Sun