TLE1 Monoklonaler Antikörper

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TLE1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F2H11

Kat-Nr. : 66216-1-PBS

Synonyme

Transducin-like enhancer protein 1, GRG1, Enhancer of split groucho-like protein 1, 2F2H11

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  WB analysis of HEK-293 using 66216-1-Ig (same clone as 66216-1-PBS)

  WB result of TLE1 antibody (66216-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLE1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66216-1-Ig (same clone as 66216-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66216-1-Ig (TLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66216-1-Ig (same clone as 66216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66216-1-Ig (same clone as 66216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66216-1-Ig (same clone as 66216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66216-1-Ig (TLE1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66216-1-Ig (same clone as 66216-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TLE1 antibody (66216-1-Ig, Clone: 2F2H11 ) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66216-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66216-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TLE1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TLE1 fusion protein Ag21327
• Vollständiger Name: transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 85-95 kDa
• GenBank-Zugangsnummer: BC010100
• Gene symbol: TLE1
• Gene ID (NCBI): 7088
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).