TLE3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TLE3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 22094-1-AP

Synonyme

HsT18976, GRG3, GRG 3, ESG3, ESG

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  WB analysis of HEK-293 using 22094-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22094-1-AP

  WB result of TLE3 antibody (22094-1-AP, 1:4000) with si-Control and si-TLE3 transfected HEK293 cells.

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  WB analysis of HEK-293 using 22094-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22094-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22094-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 22094-1-AP

  IP result of anti-TLE3 (IP:22094-1-AP, 4ug; Detection:22094-1-AP 1:1000) with HepG2 cells lysate 2400ug.

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  IHC staining of human lung cancer using 22094-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22094-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22094-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22094-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22094-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (22094-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

22094-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TLE3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TLE3 fusion protein Ag17300
• Vollständiger Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Berechnetes Molekulargewicht: 772 aa, 83 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC015729
• Gene symbol: TLE3
• Gene ID (NCBI): 7090
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES.

Publikationen

Species	Application	Title
mouse	WB,IF	Cells; Differential Regulation of TLE3 in Sertoli Cells of the Testes during Postnatal Development.; Authors - Sangho Lee ; KD Validated
		J Cell Sci; TAZ interactome analysis using nanotrap-based affinity purification-mass spectrometry; Authors - Jonathan Kelebeev