TLE3 Monoklonaler Antikörper

TLE3 Monoklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D11A8

Kat-Nr. : 66083-1-PBS

Synonyme

1D11A8, Enhancer of split groucho-like protein 3, ESG, ESG3, GRG 3

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  WB analysis of HEK-293 using 66083-1-Ig (same clone as 66083-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66083-1-Ig (same clone as 66083-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 66083-1-Ig (same clone as 66083-1-PBS)

  IP result of anti-TLE3 (IP:66083-1-Ig, 5ug; Detection:66083-1-Ig 1:500) with HEK-293 cells lysate 1480ug. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66083-1-Ig (same clone as 66083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66083-1-Ig (same clone as 66083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66083-1-PBS bindet in WB, IHC, IP, Indirect ELISA TLE3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TLE3 fusion protein Ag17906
• Vollständiger Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Berechnetes Molekulargewicht: 772 aa, 83 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC015729
• Gene symbol: TLE3
• Gene ID (NCBI): 7090
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )