Talin-1 Monoklonaler Antikörper

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• KD/KO Validated

Talin-1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2E12D10

Kat-Nr. : 67992-1-Ig

Synonyme

2E12D10, ILWEQ, KIAA1027, talin 1, talin1

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  WB analysis of HeLa using 67992-1-Ig

  WB result of Talin-1 antibody (67992-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells.

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  WB analysis using 67992-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood platelets using 67992-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 67992-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (67992-1-Ig, Clone: 2E12D10 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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  FC experiment of HeLa using 67992-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Talin-1 Monoclonal antibody (67992-1-Ig, Clone:2E12D10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67992-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Talin-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Talin-1 fusion protein Ag5937
• Vollständiger Name: talin 1
• Berechnetes Molekulargewicht: 2541 aa, 270 kDa
• Beobachtetes Molekulargewicht: 270 kDa
• GenBank-Zugangsnummer: BC042923
• Gene symbol: Talin-1
• Gene ID (NCBI): 7094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).