TLR2 Monoklonaler Antikörper

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• KD/KO Validated

TLR2 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C1D3

Kat-Nr. : 66645-1-PBS

Synonyme

CD282, TIL4, toll like receptor 2, Toll/interleukin-1 receptor-like protein 4, Toll-like receptor 2

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  WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

  WB result of TLR2 antibody (66645-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR2 transfected THP-1 cells. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 66645-1-Ig (same clone as 66645-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66645-1-Ig (TLR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66645-1-Ig (same clone as 66645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66645-1-Ig (TLR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 66645-1-Ig (same clone as 66645-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR2 antibody (66645-1-Ig, Clone: 1C1D3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66645-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50388-1

  Cytometric bead array standard curve of MP50388-1, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-2-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50388-3

  Cytometric bead array standard curve of MP50388-3, TLR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66645-1-PBS. Detection antibody: 66645-4-PBS. Standard:Ag25626. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66645-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA TLR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TLR2 fusion protein Ag25626
• Vollständiger Name: toll-like receptor 2
• Berechnetes Molekulargewicht: 784 aa, 90 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC033756
• Gene symbol: TLR2
• Gene ID (NCBI): 7097
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).