TLR3/CD283 Rekombinanter Antikörper

TLR3/CD283 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

230445G4

Rezensionen (1)

Kat-Nr. : 83136-3-RR

Synonyme

TLR3, 230445G4, CD283, toll like receptor 3, Toll-like receptor 3

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  WB analysis using 83136-3-RR

  HT-29 cells were subjected to SDS PAGE followed by western blot with 83136-3-RR (TLR3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovarian cancer using 83136-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovarian cancer using 83136-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83136-3-RR (TLR3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 83136-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 83136-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83136-3-RR (TLR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83136-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83136-3-RR against Human TLR3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: human ovarian cancer, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

83136-3-RR bindet in WB, IHC, ELISA TLR3/CD283 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TLR3/CD283 fusion protein Ag34044
• Vollständiger Name: toll-like receptor 3
• Berechnetes Molekulargewicht: 904 aa, 104 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 68 kDa
• GenBank-Zugangsnummer: BC094737
• Gene symbol: TLR3
• Gene ID (NCBI): 7098
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679). Western detected full-length (∼120 kD) and cleaved (∼70 kDa) TLR3 (PMID: 28717003).

Publikationen

Species	Application	Title
human	WB	NAR Mol Med; C G composition in transposon-derived genes is increased in FXD with perturbed immune system; Authors - Tamaki Suganuma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iustina (Verified Customer) (04-24-2025)

I have tried other Antibodies for TLR3 detection, but from all of them only this product detected the right size band: 120 kDa. Other products detected protein at 67-72 kDa, whereas with this product, I managed two bands: one at 120 and one at 67-72 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: BEAS-2B