TLR3/CD283 Rekombinanter Antikörper

TLR3/CD283 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

230445F1

Kat-Nr. : 98016-1-PBS

Synonyme

TLR3, Toll-like receptor 3, toll like receptor 3, CD283, 230445F1

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  FC experiment of human monocyte-derived immature dendritic cells using 98016-1-RR (same clone as 98016-1-PBS)

  1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 0.25 ug Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-1-RR, Clone: 230445F1) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC anti-human CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98016-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human monocyte-derived immature dendritic cells using 98016-1-RR (same clone as 98016-1-PBS)

  1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 0.25 ug Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-1-RR, Clone: 230445F1) (red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98016-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98016-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98016-1-RR against Human TLR3/CD283 were performed. The affinity constant is 5.56 nM.

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Geprüfte Anwendungen

Produktinformation

98016-1-PBS bindet in FC (Intra) TLR3/CD283 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TLR3/CD283 fusion protein Ag34044
• Vollständiger Name: toll-like receptor 3
• Berechnetes Molekulargewicht: 904 aa, 104 kDa
• GenBank-Zugangsnummer: BC094737
• Gene symbol: TLR3
• Gene ID (NCBI): 7098
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).