TLR4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TLR4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, Microtiter plate binding assay, IP, IHC, IF, CoIP, ChIP, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

422

Rezensionen (1)

Kat-Nr. : 19811-1-AP

Synonyme

ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 19811-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP (TLR4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19811-1-AP (TLR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19811-1-AP (TLR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19811-1-AP (TLR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19811-1-AP (TLR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Kolongewebe, humanes Leberkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 13 publications below
• WB: See 383 publications below
• IHC: See 35 publications below
• IF: See 44 publications below
• IP: See 7 publications below
• ELISA: See 2 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

19811-1-AP bindet in WB, Microtiter plate binding assay, IP, IHC, IF, CoIP, ChIP, Cell treatment, ELISA TLR4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Hund, Kaninchen, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TLR4 fusion protein Ag13841
• Vollständiger Name: toll-like receptor 4
• Berechnetes Molekulargewicht: 839 aa, 96 kDa
• Beobachtetes Molekulargewicht: 90-110 kDa
• GenBank-Zugangsnummer: BC117422
• Gene symbol: TLR4
• Gene ID (NCBI): 7099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLR4, also named CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described. The calculated molecular weights of the three TLR4 isoforms are 96, 91, and 73 kDa.

Publikationen

Species	Application	Title
mouse	WB	Adv Mater; Biomimetic Immunosuppressive Exosomes that Inhibit Cytokine Storms Contribute to the Alleviation of Sepsis.; Authors - Yinan Li
human	ELISA	J Clin Invest; Longistatin in tick saliva blocks advanced glycation end-product receptor activation.; Authors - Takeshi Anisuzzaman
human	WB	Gut Microbes; Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway; Authors - Jian-Wei Zhang
mouse	IF,WB	Nat Commun; TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection.; Authors - Jing Geng
mouse	WB	Nat Commun; Alarmin-painted exosomes elicit persistent antitumor immunity in large established tumors in mice.; Authors - Bingfeng Zuo ; KO Validated
mouse	IP	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sara (Verified Customer) (10-25-2020)

B cells (mouse) were lysated in RIPA buffer with protein and phosphatase inhibitors. 10 µg of protein per lane were loaded in a 10% PAGE-SDS gel. Proteins were transferred to a PVFD membrane (Turbo Blot system) and incubated overnight with 1:1000 of anti-TLR4, followed by anti-rabbit-HRP (1:10.000) on the next day. Two bands of around 70 kDa are detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary B cells from mouse spleen

TLR4 Antibody Western Blot validation (1:1000 dilution) in Primary B cells from mouse spleen (Cat no:19811-1-AP)

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