TLR4 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TLR4 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G9A4

Kat-Nr. : 66350-1-PBS

Synonyme

3G9A4, ARMD10, CD284, hToll, TOLL

---

• 

  WB analysis using 66350-1-Ig (same clone as 66350-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66350-1-Ig (same clone as 66350-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66350-1-Ig (same clone as 66350-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66350-1-Ig (same clone as 66350-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66350-1-PBS bindet in WB, IHC, ELISA TLR4 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TLR4 fusion protein Ag13857
• Vollständiger Name: toll-like receptor 4
• Berechnetes Molekulargewicht: 839 aa, 96 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC117422
• Gene symbol: TLR4
• Gene ID (NCBI): 7099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLR4, also named CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.