TLR4/CD284 Rekombinanter Antikörper

TLR4/CD284 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, FC

Konjugation

Unkonjugiert

CloneNo.

230463C7

Kat-Nr. : 98018-1-RR

Synonyme

Tlr4, CD284, toll like receptor 4, 230463C7

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  FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.

• 

  FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Balb/c peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98018-1-RR bindet in WB, FC TLR4/CD284 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TLR4/CD284 fusion protein Ag32998
• Vollständiger Name: toll-like receptor 4
• Berechnetes Molekulargewicht: 96 kDa
• GenBank-Zugangsnummer: NM_021297
• Gene symbol: Tlr4
• Gene ID (NCBI): 21898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.