TLR4/CD284 Rekombinanter Antikörper

TLR4/CD284 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

230463C7

Kat-Nr. : CL594-98018

Synonyme

CD284, Tlr4, 230463C7, toll like receptor 4

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  FC experiment of Balb/c mouse peritoneal macrophages using CL594-98018

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with CoraLite® Plus 647 Anti-Mouse CD11b (M1/70), and 0.25 ug CoraLite®594 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (CL594-98018, Clone: 230463C7) or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control Recombinant Antibody (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of Balb/c mouse peritoneal macrophages using CL594-98018

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite®594 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (CL594-98018, Clone: 230463C7) (red) or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control Recombinant Antibody (CL594-98136, Clone: 240953C9)(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Balb/c peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98018 bindet in FC TLR4/CD284 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TLR4/CD284 fusion protein Ag32998
• Vollständiger Name: toll-like receptor 4
• Berechnetes Molekulargewicht: 96 kDa
• GenBank-Zugangsnummer: NM_021297
• Gene symbol: Tlr4
• Gene ID (NCBI): 21898
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.