TLR7 Polyklonaler Antikörper

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• KD/KO Validated

TLR7 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 17232-1-AP

Synonyme

UNQ248/PRO285, Toll-like receptor 7, toll like receptor 7

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  WB analysis of mouse lung using 17232-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17232-1-AP (TLR7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17232-1-AP

  WB result of TLR7 antibody (17232-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR7 transfected Jurkat cells.

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  IHC staining of human lung using 17232-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17232-1-AP (TLR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 17232-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17232-1-AP (TLR7 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of RAW 264.7 using 17232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TLR7 antibody (17232-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

17232-1-AP bindet in WB, IHC, IF/ICC, ELISA TLR7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TLR7 fusion protein Ag11073
• Vollständiger Name: toll-like receptor 7
• Berechnetes Molekulargewicht: 1049 aa, 121 kDa
• Beobachtetes Molekulargewicht: 121 kDa, 100 kDa
• GenBank-Zugangsnummer: BC033651
• Gene symbol: TLR7
• Gene ID (NCBI): 51284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Among the TLR family, TLR7 (toll-like receptor 7) is an endosomal innate immune sensor that can detect single-stranded ribonucleic acid. Constitutive expression of TLR7 is pre-dominant in dendritic cells and B cells compared to other immune cells. Low levels of TLR7 expression have been observed in non-immune cells, such as hepatocytes, epithelial cells, and keratinocytes (PMID: 37596656).

Publikationen

Species	Application	Title
human	IHC	Nat Commun; TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase; Authors - Gang Liu
rat	WB	Acta Physiol (Oxf); Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.; Authors - Li Zhang
human	WB	Stem Cell Res Ther; Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.; Authors - Ruijie Zhang ; KD Validated
mouse	WB,IF	Mol Neurobiol; Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment; Authors - Liyun Deng
mouse	WB	Front Immunol; Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.; Authors - Lujie Yang
rat	WB	Mol Cell Endocrinol; Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.; Authors - Dongmin Li