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  • Featured Product
  • KD/KO Validated

TLR7 Polyklonaler Antikörper

TLR7 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17232-1-AP

Synonyme

TLR7, toll like receptor 7

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of mouse lung tissue using TLR7 Polyclonal antibody (17232-1-AP)

    WB analysis of mouse lung using 17232-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17232-1-AP (TLR7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TLR7 Polyclonal antibody (17232-1-AP)

    WB analysis of Jurkat using 17232-1-AP

    WB result of TLR7 antibody (17232-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLR7 transfected Jurkat cells.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using TLR7 Polyclonal antibody (17232-1-AP)

    IHC staining of human small intestine using 17232-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17232-1-AP (TLR7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using TLR7 Polyclonal antibody (17232-1-AP)

    IHC staining of human small intestine using 17232-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17232-1-AP (TLR7 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using TLR7 Polyclonal antibody (17232-1-AP)

    IF Staining of RAW 264.7 using 17232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TLR7 antibody (17232-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMauslungengewebe, Jurkat-Zellen
Erfolgreiche Detektion in IHChumanes Dünndarmgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFRAW 264.7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17232-1-AP bindet in WB, IHC, IF, ELISA TLR7 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TLR7 fusion protein Ag11073
Vollständiger Name toll-like receptor 7
Berechnetes Molekulargewicht 1049 aa, 121 kDa
Beobachtetes Molekulargewicht 121 kDa, 100 kDa
GenBank-ZugangsnummerBC033651
Gene symbol TLR7
Gene ID (NCBI) 51284
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for TLR7 antibody 17232-1-APProtokoll herunterladen
IHC protocol for TLR7 antibody 17232-1-APProtokoll herunterladen
IF protocol for TLR7 antibody 17232-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Nat Commun

TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase

Authors - Gang Liu
View Article
ratWB

Acta Physiol (Oxf)

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Authors - Li Zhang
View Article
humanWB

Stem Cell Res Ther

Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.

Authors - Ruijie Zhang
  • KD Validated
View Article
mouseWB,IF

Mol Neurobiol

Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment

Authors - Liyun Deng
View Article
mouseWB

Front Immunol

Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.

Authors - Lujie Yang
View Article
ratWB

Mol Cell Endocrinol

Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.

Authors - Dongmin Li
View Article