TLR8 Monoklonaler Antikörper

TLR8 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B6A4

Kat-Nr. : 67317-1-PBS

Synonyme

CD288, TLR8, toll like receptor 8

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  WB analysis using 67317-1-Ig (same clone as 67317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 67317-1-Ig (same clone as 67317-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67317-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA TLR8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TLR8 fusion protein Ag16566
• Vollständiger Name: toll-like receptor 8
• Berechnetes Molekulargewicht: 1041 aa, 120 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC101075
• Gene symbol: TLR8
• Gene ID (NCBI): 51311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.