TLR9 Polyklonaler Antikörper

TLR9 Polyklonal Antikörper für IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 17230-1-AP

Synonyme

CD289, toll like receptor 9, Toll-like receptor 9, UNQ5798/PRO19605

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  IP experiment of Raji using 17230-1-AP

  IP result of anti-TLR9 (IP:17230-1-AP, 4ug; Detection:17230-1-AP 1:500) with Raji cells lysate 1120 ug.

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Geprüfte Anwendungen

• Erfolgreiche IP: Raji-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

17230-1-AP bindet in WB, IP, ELISA TLR9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TLR9 fusion protein Ag11071
• Vollständiger Name: toll-like receptor 9
• Berechnetes Molekulargewicht: 1032 aa, 116 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 60 kDa
• GenBank-Zugangsnummer: BC032713
• Gene symbol: TLR9
• Gene ID (NCBI): 54106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLR9, also known as CD289, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents and mediate the production of cytokines necessary for the development of effective immunity. TLR9 is a nucleotide-sensing TLR that is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PMID:14716310). Upon CpG stimulation, it induces B-cell proliferation, activation, survival, and antibody production (PMID:23857366). TLR9 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and the inflammatory response (PMID:11564765, 17932028).

Publikationen

Species	Application	Title
rat	WB	Mol Cell Endocrinol; Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.; Authors - Dongmin Li