TMP21 Monoklonaler Antikörper

TMP21 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3B11F9

Kat-Nr. : 67876-1-PBS

Synonyme

TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23

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  WB analysis using 67876-1-Ig (same clone as 67876-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  WB analysis using 67876-1-Ig (same clone as 67876-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMP21 antibody (67876-1-Ig, Clone: 3B11F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67876-1-Ig (same clone as 67876-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug TMP21 Monoclonal antibody (67876-1-Ig, Clone:3B11F9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67876-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA TMP21 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TMP21 fusion protein Ag7516
• Vollständiger Name: transmembrane emp24-like trafficking protein 10 (yeast)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC001825
• Gene symbol: TMED10
• Gene ID (NCBI): 10972
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.