TMED2 Polyklonaler Antikörper

TMED2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 11981-1-AP

Synonyme

RNP24, Membrane protein p24A, p24, p24 family protein beta-1, p24beta1

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  WB analysis using 11981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11981-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 11981-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11981-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11981-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11981-1-AP (TMED2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 11981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11981-1-AP (TMED2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 11981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11981-1-AP (TMED2 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Mauslebergewebe, Maus-Pankreasgewebe, Maushautgewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11981-1-AP bindet in WB, IHC, IF, ELISA TMED2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMED2 fusion protein Ag2607
• Vollständiger Name: transmembrane emp24 domain trafficking protein 2
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC025957
• Gene symbol: TMED2
• Gene ID (NCBI): 10959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transmembrane emp24 domain-containing protein 2 (TMED2) belongs to the p24 receptor family, being mainly involved in vesicular trafficking of proteins between the endoplasmic reticulum and Golgi apparatus.

Publikationen

Species	Application	Title
human	WB	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li
rat	WB	PLoS Biol; TMED9 coordinates the clearance of misfolded GPI-anchored proteins out of the ER and into the Golgi; Authors - Elsa Ronzier
human	WB	BMC Biol; ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.; Authors - Xihua Yue
human	WB,IF	BMC Cancer; The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.; Authors - Xue Yang
human	WB	Adv Sci (Weinh); TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.; Authors - Hak Park
human	WB	Ann Transl Med; Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer; Authors - Zheng Fang ; KD Validated