TMED3 Polyklonaler Antikörper

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• KD/KO Validated

TMED3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 21902-1-AP

Synonyme

Transmembrane emp24 domain-containing protein 3, p26, p24gamma4, p24 family protein gamma-4, Membrane protein p24B

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  WB analysis using 21902-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 21902-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21902-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 21902-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 21902-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21902-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 21902-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21902-1-AP (TMED3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, HT-1080.Zellen, MCF-7-Zellen, Maushautgewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below

Produktinformation

21902-1-AP bindet in WB, IHC, ELISA TMED3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMED3 fusion protein Ag14032
• Vollständiger Name: transmembrane emp24 protein transport domain containing 3
• Berechnetes Molekulargewicht: 217 aa, 25 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC000027
• Gene symbol: TMED3
• Gene ID (NCBI): 23423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
human	WB	J Biol Chem; Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.; Authors - Chengfeng Merriman
human	WB	Toxicol Appl Pharmacol; TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT.; Authors - Danjie Zhang ; KD Validated
human	WB	Onco Targets Ther; TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling.; Authors - Xiumei Zhang ; KD Validated
human	WB	Ann Transl Med; Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer; Authors - Zheng Fang ; KD Validated
human	WB	J Cancer Res Clin Oncol; Analysis and Validation of TMED3 correlates with poor prognosis and tumor immune infiltration of glioma; Authors - Gang Liao