TMED9 Polyklonaler Antikörper

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TMED9 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21620-1-PBS

Synonyme

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  WB analysis using 21620-1-AP (same clone as 21620-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21620-1-AP (same clone as 21620-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 21620-1-AP (same clone as 21620-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 21620-1-AP (same clone as 21620-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 21620-1-AP (same clone as 21620-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 21620-1-AP (same clone as 21620-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21620-1-AP (TMED9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 21620-1-AP (same clone as 21620-1-PBS)

  IP result of anti-TMED9 (IP:21620-1-AP, 4ug; Detection:21620-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21620-1-AP (TMED9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21620-1-AP (same clone as 21620-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 21620-1-AP (TMED9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21620-1-AP (same clone as 21620-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMED9 antibody (21620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 21620-1-AP (same clone as 21620-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TMED9 antibody (21620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 21620-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21620-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA TMED9 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMED9 fusion protein Ag13813
• Vollständiger Name: transmembrane emp24 protein transport domain containing 9
• Berechnetes Molekulargewicht: 235 aa, 27 kDa
• Beobachtetes Molekulargewicht: 24-27 kDa
• GenBank-Zugangsnummer: BC001123
• Gene symbol: TMED9
• Gene ID (NCBI): 54732
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).