TMEM115 Polyklonaler Antikörper

TMEM115 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25536-1-AP

Synonyme

Transmembrane protein 115, Placental protein 6, PL6, LUCA11.2

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  WB analysis of U2OS using 25536-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 25536-1-AP (TMEM115 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 25536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25536-1-AP (TMEM115 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 25536-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 25536-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 25536-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 25536-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25536-1-AP (TMEM115 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25536-1-AP bindet in WB, IHC, ELISA TMEM115 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM115 fusion protein Ag22258
• Vollständiger Name: transmembrane protein 115
• Berechnetes Molekulargewicht: 351 aa, 38 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC017367
• Gene symbol: TMEM115
• Gene ID (NCBI): 11070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM115 also known as PL6, located on chromosome 3p21.3, was originally thought to function as a tumor suppressor. TMEM115 is an integral membrane protein of the Golgi complex involved in retrograde transport.