TMEM119 Polyklonaler Antikörper

TMEM119 Polyklonal Antikörper für IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27585-1-PBS

Synonyme

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF

---

• 

  IHC staining of mouse brain using 27585-1-AP (same clone as 27585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27585-1-AP (TMEM119 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 27585-1-AP (same clone as 27585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM119 antibody (27585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 27585-1-AP (same clone as 27585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using TMEM119 antibody (27585-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 27585-1-AP (same clone as 27585-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug TMEM119 Polyclonal antibody (27585-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

27585-1-PBS bindet in IHC, IF-P, FC (Intra), ELISA TMEM119 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM119 fusion protein Ag26269
• Vollständiger Name: transmembrane protein 119
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_181724
• Gene symbol: TMEM119
• Gene ID (NCBI): 338773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP.