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TMEM119 Monoklonaler Antikörper
TMEM119 Monoklonal Antikörper für WB, ELISA
Wirt / Isotyp
Maus / IgG3
Getestete Reaktivität
Hausschwein, human, Kaninchen, Maus, Ratte
Anwendung
WB, ELISA
Konjugation
Unkonjugiert
CloneNo.
1E7A2
Kat-Nr. : 66948-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Kaninchenhirngewebe, Hühnerhirngewebe, Maushirngewebe, Maus-Cerebellum-Gewebe, Hausschwein-Hirngewebe, Hausschwein-Cerebellum-Gewebe, Kaninchen-Cerebellum-Gewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 17 publications below |
| FC | See 1 publications below |
Produktinformation
66948-1-Ig bindet in WB, ELISA TMEM119 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Kaninchen, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG3 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | TMEM119 fusion protein Ag26286 |
| Vollständiger Name | transmembrane protein 119 |
| Berechnetes Molekulargewicht | 29 kDa |
| Beobachtetes Molekulargewicht | 52 kDa |
| GenBank-Zugangsnummer | NM_181724 |
| Gene symbol | TMEM119 |
| Gene ID (NCBI) | 338773 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#66948-1-Ig. The predicted MW of TMEM119 is 29 kDa. Catalog#66948-1-Ig recognizes 50 kDa band may due to glycosylation.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for TMEM119 antibody 66948-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
ACS Nano Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia | ||
J Extracell Vesicles Characterization of brain-derived extracellular vesicles reveals changes in cellular origin after stroke and enrichment of the prion protein with a potential role in cellular uptake. | ||
Int J Biol Sci Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice | ||
Acta Pharmacol Sin Prebiotic diet normalizes aberrant immune and behavioral phenotypes in a mouse model of autism spectrum disorder | ||
Oxid Med Cell Longev Methane-Rich Saline Alleviates CA/CPR Brain Injury by Inhibiting Oxidative Stress, Microglial Activation-Induced Inflammatory Responses, and ER Stress-Mediated Apoptosis. | ||
Antioxidants (Basel) TNF-α Levels Are Increased in Patients with Subjective Cognitive Impairment and Are Negatively Correlated with β Amyloid-42 |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Amirah (Verified Customer) (03-02-2023) | The antibody works using the antigen retrieval method beforehand and using normal goat serum as a blocking buffer. Images not perfect but it still allows the antibody to be expressed.
|
FH Hannes (Verified Customer) (03-08-2021) | The antibody did not work in IHC-p on human and murine brain tissue.
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