TMEM126A Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

TMEM126A Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240425A11

Kat-Nr. : 83440-2-PBS

Synonyme

240425A11, OPA7, TMEM, TMEM126

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  WB analysis using 83440-2-RR (same clone as 83440-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83440-2-RR (TMEM126A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 83440-2-RR (same clone as 83440-2-PBS)

  WB result of TMEM126A antibody (83440-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM126A transfected HeLa cells. This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83440-2-RR (same clone as 83440-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM126A antibody (83440-2-RR, Clone: 240425A11 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83440-2-RR (same clone as 83440-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TMEM126A Recombinant antibody (83440-2-RR, Clone:240425A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00449-1

  Cytometric bead array standard curve of MP00449-1, TMEM126A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83440-2-PBS. Detection antibody: 83440-1-PBS. Standard: Ag14705. Range: 6.25-100 ng/mL.

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  Affinity and Kinetic Characterization of 83440-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83440-2-RR against Human TMEM126A were performed. The affinity constant is 0.589 nM.

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Geprüfte Anwendungen

Produktinformation

83440-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA TMEM126A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TMEM126A fusion protein Ag14705
• Vollständiger Name: transmembrane protein 126A
• Berechnetes Molekulargewicht: 195 aa, 22 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC007875
• Gene symbol: TMEM126A
• Gene ID (NCBI): 84233
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM126A is a mitochondrial inner membrane protein that is enriched in the cristae. TMEM126A is necessary for Complex I assembly or stability.Complex I (CI) is the largest enzyme of the mitochondrial respiratory chain, and its defects are the main cause of mitochondrial disease.