TMEM165 Polyklonaler Antikörper

TMEM165 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20485-1-PBS

Synonyme

Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165, Transmembrane protein PT27

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  WB analysis of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  IP result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20485-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA TMEM165 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM165 fusion protein Ag14362
• Vollständiger Name: transmembrane protein 165
• Berechnetes Molekulargewicht: 324 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC003545
• Gene symbol: TMEM165
• Gene ID (NCBI): 55858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT), a member of methyltransferase superfamily, is an enzyme involved in the normal metabolic inactivation of thiopurine drugs such as azathioprine, 6-mercaptopurine and 6-thioguanine. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Defects in the TPMT gene leads to decreased methylation and decreased inactivation of 6MP leading to enhanced bone marrow toxicity which may cause myelosuppression, anemia, bleeding tendency, leukopenia and infection.