TMEM165 Rekombinanter Antikörper

TMEM165 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230517B1

Kat-Nr. : 83146-1-PBS

Synonyme

230517B1, Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165

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  IF Staining of HepG2 using 83146-1-RR (same clone as 83146-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM165 antibody (83146-1-RR, Clone: 230517B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83146-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83146-1-RR (same clone as 83146-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM165 Recombinant antibody (83146-1-RR, Clone:230517B1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83146-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00217-2

  Cytometric bead array standard curve of MP00217-2, TMEM165 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83146-2-PBS. Detection antibody: 83146-1-PBS. Standard: Ag14362. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83146-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83146-1-RR against Human TMEM165 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83146-1-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA TMEM165 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TMEM165 fusion protein Ag14362
• Vollständiger Name: transmembrane protein 165
• Berechnetes Molekulargewicht: 324 aa, 35 kDa
• GenBank-Zugangsnummer: BC003545
• Gene symbol: TMEM165
• Gene ID (NCBI): 55858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.