TMEM173/STING Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TMEM173/STING Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(45)

CloneNo.

1F1E1

Rezensionen (1)

Kat-Nr. : 66680-1-Ig

Synonyme

STING, NET23, MPYS, MITA, ERIS

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• 

  WB analysis using 66680-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of THP-1 using 66680-1-Ig

  WB result of TMEM173/STING antibody (66680-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells.

• 

  WB analysis of HSC-T6 using 66680-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66680-1-Ig

  WB result of TMEM173/STING antibody (66680-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected HEK-293 cells.

• 

  WB analysis of HepG2 using 66680-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66680-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66680-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 66680-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 66680-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66680-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TMEM173/STING antibody (66680-1-Ig, Clone: 1F1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MOLT-4-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 34 publications below
• IHC: See 6 publications below
• IF: See 18 publications below
• IP: See 2 publications below

Produktinformation

66680-1-Ig bindet in WB, IHC, IF-P, IP, ELISA TMEM173/STING und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TMEM173/STING fusion protein Ag20363
• Vollständiger Name: transmembrane protein 173
• Berechnetes Molekulargewicht: 379 aa, 42 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC047779
• Gene symbol: STING
• Gene ID (NCBI): 340061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

Publikationen

Species	Application	Title
human	WB, IF	Immunity; Golgi apparatus-synthesized sulfated glycosaminoglycans mediate polymerization and activation of the cGAMP sensor STING.; Authors - Run Fang
mouse	WB	Cell Death Differ; The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson's disease.; Authors - Miao Zhao ; KO Validated
mouse	WB	Cell Death Differ; Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.; Authors - Ming Zou
human		J Nanobiotechnology; Platelet hitchhiking vascular-disrupting agents for self-amplified tumor-targeting therapy; Authors - Hongyu Chu
human,mouse	IF	Autophagy; Lipotoxicity-induced STING1 activation stimulates MTORC1 and restricts hepatic lipophagy.; Authors - Kunpeng Liu ; KO Validated
mouse	IF	J Neuroinflammation; The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage; Authors - Xingfei Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Will (Verified Customer) (04-25-2019)

good antibody, will order again

• Applications: Western Blot,