TMEM173/STING Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TMEM173/STING Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen

1

Kat-Nr. : CL594-19851

Synonyme

FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, STING, TMEM173, transmembrane protein 173

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Galerie der Validierungsdaten

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  IF Staining of HT-29 using CL594-19851

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-19851

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-19851

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-19851

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-19851

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TMEM173/STING (CL594-19851) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of THP-1 using CL594-19851

  1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human TMEM173/STING (CL594-19851) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of NIH/3T3 using CL594-19851

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human TMEM173/STING (CL594-19851) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HT-29-Zellen, HepG2-Zellen, humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: THP-1-Zellen, HepG2-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-19851 bindet in IF, FC (Intra) TMEM173/STING und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM173/STING fusion protein Ag13921
• Vollständiger Name: transmembrane protein 173
• Berechnetes Molekulargewicht: 379 aa, 42 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa, 80 kDa
• GenBank-Zugangsnummer: BC047779
• Gene symbol: TMEM173
• Gene ID (NCBI): 340061
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

Publikationen

Species	Application	Title
mouse	IF	J Neuroinflammation; The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage; Authors - Xingfei Zhu

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C