TMEM175 Polyklonaler Antikörper

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• KD/KO Validated

TMEM175 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 19925-1-AP

Synonyme

Transmembrane protein 175, hTMEM175

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  WB analysis using 19925-1-AP

  Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

19925-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA TMEM175 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM175 fusion protein Ag13890
• Vollständiger Name: transmembrane protein 175
• Berechnetes Molekulargewicht: 504 aa, 56 kDa
• Beobachtetes Molekulargewicht: 41-45 kDa, 54-60 kDa
• GenBank-Zugangsnummer: BC005158
• Gene symbol: TMEM175
• Gene ID (NCBI): 84286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.

Publikationen

Species	Application	Title
	WB	Nature; A growth-factor-activated lysosomal K+ channel regulates Parkinson's pathology.; Authors - Jinhong Wie ; KO Validated
human	WB	Cell; TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal Function.; Authors - Chunlei Cang
human	WB	Ann Neurol; Genetic, Structural, and Functional Evidence Link TMEM175 to Synucleinopathies.; Authors - Lynne Krohn
human	WB	Proc Natl Acad Sci U S A; TMEM175 deficiency impairs lysosomal and mitochondrial function and increases α-synuclein aggregation.; Authors - Sarah Jinn ; KO Validated
mouse	WB	Mol Med; Modulation of anti-cardiac fibrosis immune responses by changing M2 macrophages into M1 macrophages; Authors - Shiqi Chen
		Hum Mol Genet; Mitochondrial clearance and maturation of autophagosomes are compromised in LRRK2 G2019S familial Parkinson's disease patient fibroblasts.; Authors - Joanna A Korecka