TMEM175 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

TMEM175 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241567B9

Kat-Nr. : 84554-1-RR

Synonyme

241567B9, hTMEM175, Transmembrane protein 175

---

• 

  WB analysis of HepG2 using 84554-1-RR

  WB result of TMEM175 antibody (84554-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells.

• 

  WB analysis using 84554-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84554-1-RR (TMEM175 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84554-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84554-1-RR against Human TMEM175 were performed. The affinity constant is 5.17 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84554-1-RR bindet in WB, ELISA TMEM175 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TMEM175 fusion protein Ag13890
• Vollständiger Name: transmembrane protein 175
• Berechnetes Molekulargewicht: 504 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC005158
• Gene symbol: TMEM175
• Gene ID (NCBI): 84286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.