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TMEM26 Polyklonaler Antikörper

TMEM26 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27267-1-PBS

Synonyme

transmembrane protein 26

Filter:
  • Western Blot (WB) analysis of various lysates using TMEM26 Polyclonal antibody (27267-1-AP)

    WB analysis using 27267-1-AP (same clone as 27267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27267-1-AP (TMEM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27267-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM26 Polyclonal antibody (27267-1-AP)

    WB analysis using 27267-1-AP (same clone as 27267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27267-1-AP (TMEM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27267-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27267-1-PBS bindet in WB, Indirect ELISA TMEM26 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TMEM26 fusion protein Ag24409
Vollständiger Name transmembrane protein 26
Berechnetes Molekulargewicht 368 aa, 42 kDa
Beobachtetes Molekulargewicht50 kDa
GenBank-ZugangsnummerBC042872
Gene symbol TMEM26
Gene ID (NCBI) 219623
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Transmembrane protein 26 (TMEM26) gene encodes a protein containing multiple transmembrane fragments. Immunostaining indicated elevated TMEM26 expression in ESCC tumors. Various ESCC cell lines showed a high TMEM26 expression, where its plasma membrane localization was confirmed. The RNAi depletion of TMEM26 in TMEM26-high ESCC cells suppressed EMT-related alterations, including invasion, migration, and marker gene expression. Conversely, TMEM26 overexpression in TMEM26-low ESCC cells promoted these EMT-related alterations. Interestingly, TMEM26 depletion or overexpression did not affect cell growth, indicating its specific involvement in the EMT process. The animal study demonstrated the contributive role of TMEM26 in metastatic ESCC. Mechanistically, TMEM26 promoted NF-κB signaling to accelerate EMT in ESCC cells. The plasma membrane presentation and TJ protein assembly were impaired by TMEM26, which was likely to be another mechanism for EMT regulation by TMEM26 in ESCC. Therefore, TMEM26 disrupted TJ formation and promoted NF-κB signaling during the EMT activation in ESCC (PMID: 37611445). TMEM26 shows features for chain, glycosylation, modified residue (large scale data).