TMEM41B Polyklonaler Antikörper

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• KD/KO Validated

TMEM41B Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29270-1-PBS

Synonyme

Transmembrane protein 41B, KIAA0033

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  WB analysis of HeLa using 29270-1-AP (same clone as 29270-1-PBS)

  WB result of TMEM41B antibody (29270-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM41B transfected HeLa cells. This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

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  WB analysis using 29270-1-AP (same clone as 29270-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29270-1-AP (TMEM41B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 29270-1-AP (same clone as 29270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 29270-1-AP (same clone as 29270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29270-1-AP (same clone as 29270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29270-1-AP (same clone as 29270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 29270-1-AP (same clone as 29270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 29270-1-AP (same clone as 29270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29270-1-PBS bindet in WB, IHC, Indirect ELISA TMEM41B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM41B fusion protein Ag30810
• Vollständiger Name: transmembrane protein 41B
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 22-28 kDa
• GenBank-Zugangsnummer: BC035034
• Gene symbol: TMEM41B
• Gene ID (NCBI): 440026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transmembrane protein 41B (TMEM41B), also known as Stasimon, is a major target of survival motor neuron (SMN)-dependent U12 splicing and has been identified as a protein required for motor circuit function (PMID: 23063131). TMEM41B localizes to the endoplasmic reticulum. It is involved in autophagosome biogenesis and lipid mobilization (PMID: 30126924). TMEM41B has been shown to be essential for mouse embryonic development (PMID: 30352685). Additionally, genome-scale CRISPR knockout screens reveal that TMEM41B is required for infection of flavivirus and coronavirus, including SARS-CoV-2 (PMID: 33052348; 33052332). Alternative splicing results in three transcript variants encoding distinct isoforms.