TMEM50B Polyklonaler Antikörper

TMEM50B Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15941-1-AP

Synonyme

C21orf4, HCVP7TP3, TMEM50B, transmembrane protein 50B

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 15941-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15941-1-AP (TMEM50B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15941-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15941-1-AP (TMEM50B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15941-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15941-1-AP (TMEM50B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15941-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15941-1-AP (TMEM50B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15941-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15941-1-AP (TMEM50B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15941-1-AP bindet in WB, IHC, ELISA TMEM50B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM50B fusion protein Ag8220
• Vollständiger Name: transmembrane protein 50B
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 14 kDa, 30 kDa, 50 kDa
• GenBank-Zugangsnummer: BC000569
• Gene symbol: TMEM50B
• Gene ID (NCBI): 757
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM50B, originally referred to as C21orf4, is a predicted transmembrane protein, estimated to contain four transmembrane-spanning domains with both the carboxy- and amino-terminals being cytoplasmic (PMID: 10077530; 18541381). Human TMEM50B shares sequence homologies of around 90-100% with mouse and rat Tmem50b. In a Down syndrome mouse model, Ts1Cje, Tmem50b is shown to be one of the few genes significantly over-expressed during cerebellar development (PMID: 15590701). It has been reported that rodent Tmem50b is a developmentally-regulated intracellular ER and Golgi apparatus membrane protein that may prove important for correct brain development through functions associated with precursor cells and glia (PMID: 18541381). TMEM50B can exist as a dimer and a trimer conformation (PMID: 18541381).