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TMEM53 Rekombinanter Antikörper

TMEM53 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240511C3

Kat-Nr. : 83554-3-PBS

Synonyme

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

Filter:
  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TMEM53 Recombinant antibody (83554-3-RR)

    IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TMEM53 Recombinant antibody (83554-3-RR)

    IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using TMEM53 Recombinant antibody (83554-3-RR)

    FC experiment of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug TMEM53 Recombinant antibody (83554-3-RR, Clone:240511C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83554-3-RR

    Affinity and Kinetic Characterization of 83554-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83554-3-RR against Human TMEM53 were performed. The affinity constant is 44.5 nM.

Geprüfte Anwendungen

Produktinformation

83554-3-PBS bindet in IF/ICC, FC (Intra), ELISA TMEM53 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen TMEM53 fusion protein Ag21321
Vollständiger Name transmembrane protein 53
Berechnetes Molekulargewicht 277 aa, 32 kDa
GenBank-ZugangsnummerBC064520
Gene symbol TMEM53
Gene ID (NCBI) 79639
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.