TMEM53 Rekombinanter Antikörper

TMEM53 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240511C3

Kat-Nr. : 83554-3-RR

Synonyme

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

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  IF Staining of A549 using 83554-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 83554-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  FC experiment of A549 using 83554-3-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug TMEM53 Recombinant antibody (83554-3-RR, Clone:240511C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83554-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83554-3-RR against Human TMEM53 were performed. The affinity constant is 44.5 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83554-3-RR bindet in IF/ICC, FC (Intra), ELISA TMEM53 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TMEM53 fusion protein Ag21321
• Vollständiger Name: transmembrane protein 53
• Berechnetes Molekulargewicht: 277 aa, 32 kDa
• GenBank-Zugangsnummer: BC064520
• Gene symbol: TMEM53
• Gene ID (NCBI): 79639
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM53 was initially described as a nuclear envelope transmembrane (NET) protein because of its nuclear envelope localization. Research has found that TMEM53 functionality does not rely on viral RNA binding or canonical IFN responses, and TMEM53 exerts an antiviral effect on other bat HKU2-related CoVs in a species-specific manner. Therefore, TMEM53 may be a potential therapeutic target for HKU2-related CoV infections and useful for preparing for future outbreaks of this viral species. (PMID: 37584551)