TMEM53 Rekombinanter Antikörper

TMEM53 Rekombinant Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240511C3

Kat-Nr. : CL488-83554-3

Synonyme

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

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  IF Staining of A549 using CL488-83554-3

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 TMEM53 antibody (CL488-83554-3, Clone: 240511C3 ) at dilution of 1:200.

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  FC experiment of A549 using CL488-83554-3

  1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated TMEM53 Recombinant antibody (CL488-83554-3, Clone:240511C3)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-83554-3 bindet in IF/ICC, FC (Intra) TMEM53 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TMEM53 fusion protein Ag21321
• Vollständiger Name: transmembrane protein 53
• Berechnetes Molekulargewicht: 277 aa, 32 kDa
• GenBank-Zugangsnummer: BC064520
• Gene symbol: TMEM53
• Gene ID (NCBI): 79639
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM53 was initially described as a nuclear envelope transmembrane (NET) protein because of its nuclear envelope localization. Research has found that TMEM53 functionality does not rely on viral RNA binding or canonical IFN responses, and TMEM53 exerts an antiviral effect on other bat HKU2-related CoVs in a species-specific manner. Therefore, TMEM53 may be a potential therapeutic target for HKU2-related CoV infections and useful for preparing for future outbreaks of this viral species. (PMID: 37584551)