TMEM59 Rekombinanter Antikörper

TMEM59 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240457A1

Kat-Nr. : 83656-3-PBS

Synonyme

UNQ169/PRO195, Liver membrane-bound protein, HSPC001, C1orf8, 240457A1

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  IF Staining of HepG2 using 83656-3-RR (same clone as 83656-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMEM59 antibody (83656-3-RR, Clone: 240457A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83656-3-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83656-3-RR (same clone as 83656-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM59 Recombinant antibody (83656-3-RR, Clone:240457A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83656-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83656-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83656-3-RR against Human TMEM59 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83656-3-PBS bindet in IF/ICC, FC (Intra), ELISA TMEM59 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TMEM59 fusion protein Ag1968
• Vollständiger Name: transmembrane protein 59
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC016374
• Gene symbol: TMEM59
• Gene ID (NCBI): 9528
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.