TMEM65 Polyklonaler Antikörper

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• KD/KO Validated

TMEM65 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21913-1-PBS

Synonyme

Transmembrane protein 65

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• 

  WB analysis using 21913-1-AP (same clone as 21913-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21913-1-AP (same clone as 21913-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 21913-1-AP (same clone as 21913-1-PBS)

  WB result of TMEM65 antibody (21913-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HEK-293 cells. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 21913-1-AP (same clone as 21913-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Huh-7 using 21913-1-AP (same clone as 21913-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Huh-7 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21913-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TMEM65 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM65 fusion protein Ag15509
• Vollständiger Name: transmembrane protein 65
• Berechnetes Molekulargewicht: 240 aa, 26 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC032396
• Gene symbol: TMEM65
• Gene ID (NCBI): 157378
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.