TMEM87A Polyklonaler Antikörper

TMEM87A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 25091-1-AP

Synonyme

Transmembrane protein 87A, TM87A, PSEC0094, Elkin1

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  WB analysis using 25091-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 25091-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25091-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 25091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 25091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 25091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 25091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, MDA-MB-453s-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25091-1-AP bindet in WB, IHC, ELISA TMEM87A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM87A fusion protein Ag18715
• Vollständiger Name: transmembrane protein 87A
• Berechnetes Molekulargewicht: 555 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC005335
• Gene symbol: TMEM87A
• Gene ID (NCBI): 25963
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a band around 60 kd, however, this band is not sensitive to deglycosylation. What's worse, this band also occurred in solo loading buffer.

• Applications: Western Blot